UJI EFEKTIVITAS EKSTRAK BIJI KETUMBAR (CORIANDRUM SATIVUM L.) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL TIKUS HIPERKOLESTEROLEMIA DIABETES
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Abstract
Diabetes Mellitus (DM) is metabolic disease characterized hyperglicemia caused by abnormalities of insulin secretion, insulin sensitivity, or both of them. Persistent hyperglicemia can trigger the production of oxidative stress. Oxidative stress can cause glomerular hypertrophy. Coriander seed extract has the potential to overcome oxidative stress. This study aims to determine the effect of coriander seed extract to kidney histopathological examination, blood cholesterol, and blood sugar level in hypercholestrolemia diabetic rats. Samples consist of 30 males wistar rats were divided into five treatments i.e: (K1) standard feed and aquades, (K2) high-fat feed, alloxan, and glibenclamid 0,045 mg/day, (K3) high-fat feed, alloxan, and coriander seed extract 300 mg/kgBW/day, (K4) high-fat feed, alloxan, and coriander seed extract 500mg/kgBW/day, (K5) high-fat feed, alloxan, and coriander seed extract 700mg/kgBW/day. Coriander seed extract was given for 28 days. The result of Wilcoxon test showed that coriander seed extract can reduce blood cholesterol level in diabetic hypercholesterolemia, meanwhile the result of paired T-test showed that it can reduce blood sugar level. Measurement of kidney’s histopathological structure as glomerular area was analyzed by Kruskal Wallis test (p=0,001) and continued with Mann-Whitney test. Group K4 can reduce glomerular area significantly compared with group K3 and K5. In conclussion, coriander seed extract can reduce blood cholesterol, blood sugar level and effectively reduce glomerular hypertrophy at dose of 500mg/kgBW/day.
Keyword : Coriandrum sativum L.; coriander seed extract; glomerular hypertrophy; blood cholesterol level; blood glucose level
ABSTRAK
Diabetes Melitus (DM) merupakan suatu penyakit metabolik dengan karakteristik hiperglikemi yang terjadi karena kelainan sekresi insulin, kerja insulin atau kedua-duanya. Hiperglikemi persisten dapat memicu produksi stres oksidatif. Stres oksidatif dapat menyebabkan terjadinya hipertrofi glomerulus. Ekstrak biji ketumbar (Coriandrum sativum L.) berpotensi mengatasi stres oksidatif. Penelitian ini bertujuan untuk mengetahui efek pemberian esktrak biji ketumbar (Coriandrum sativum L.) terhadap gambaran histopatologi ginjal, kadar kolesterol darah, dan kadar gula darah tikus model hiperkolesterolemia diabetes. Sebanyak 30 ekor tikus putih jantan galur wistar, dikelompokkan menjadi lima kelompok dengan perlakuan berbeda yaitu: (K1) pakan standar dan aquades, (K2) pakan tinggi lemak, aloksan, dan glibenklamid 0,045mg/hari, (K3) pakan tinggi lemak, aloksan dan ekstrak biji ketumbar 300mg/kgBB/hari, K4) pakan tinggi lemak, aloksan dan ekstrak biji ketumbar 500mg/kgBB/hari, (K5) pakan tinggi lemak, aloksan, dan ekstrak biji ketumbar 700mg/kgBB/hari. Ekstrak biji ketumbar diberikan selama 28 hari. Hasil uji Wilcoxon menunjukkan ekstrak biji ketumbar dapat menurunkan kadar kolesterol darah, sedangkan hasil uji T berpasangan menunjukkan ekstrak biji ketumbar dapat menurunkan kadar guka darah sewaktu pada tikus model hiperkolesterolemia diabetes. Histopatologi ginjal dianalisis menggunakan uji Kruskal Wallis didapatkan (p=0,001) dan dilanjutkan dengan uji Mann-Whitney. Pada kelompok K4 terdapat penurunan luas glomerulus yang signifikan dibanding kelompok K3 dan K5. Kesimpulannya, esktrak biji ketumbar dapat menurunkan kadar kolesterol darah, kadar gula darah, dan pada dosis 500mg/kgBB/hari paling efektif untuk menurunkan hipertrofi gomerulus.
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References
Dahlan, Sopiyudin. (2014). Statistik Untuk Kedokteran dan Kesehatan Edisi 6. Salmba Medika. Jakarta.
Dvm, MGP. (2016). Saunders Handbook of Veterinary Drugs Small and Large Animal, 4th edn. ELSEVIER.
Gross, JL, Azevedo, MJD, Silveiro, SP, Canani, LH, Caramori, ML, Zelmanovitz, T. (2005). Kaplow Shavell Fairness v Welfare Chapter 3.pdf. Diabetic Nephropathy : Diagnosis, Prevention, and Treatment. Diakses 10 Juli 2019. Available at : https://doi.org/10.2337/diacare.28.1.164.
Hao, M, Head, WS, Gunawardana, SC, Hasty, AH, Piston, DW. (2007). A Novel Mechanism for Pancreatic ?Cell Dysfunction’, 56(September). Diakses 31 Julia 2019. doi: 10.2337/db07-0056.2-DG.
Joshi, SC, Sharma, N, Sharma, P. (2012). Antioxidant and lipid lowering effects of Coriandrum sativum in cholesterol fed rabbits. International Journal of Pharmacy and Pharmaceutical Sciences, 4(SUPPL.3), pp. 231–234. Diakses 28 Januari 2020. Available at: https://www.researchgate.net/publication/267037167_Antioxidant_and_lipid_lowering_effects_of_Coriandrum_sativum_in_cholesterol_fed_rabbits
Kajal, A & Singh, R. (2019). Coriandrum sativum seeds extract mitigate progression of diabetic nephropathy in experimental rats via AGEs inhibition, PLoS ONE, 14(3), pp. 1–13. Diakses 1 Juli 2019. doi: 10.1371/journal.pone.0213147
Kasper, DL, Hauser, SL, Jameson, JL, Fauci, AS, Longo, DL, Loscalzo, J. (2015). Harrison’s Principles of Internal Medicine, 19th edn. McGraw Hill. USA.
Kisaoglu, A, Borekci, B, Yapca, OE, Bilen, H, Suleyman, H. (2013). Tissue Damage and Oxidant/Antioxidant Balance, The Eurasian Journal of Medicine, 45(1), pp. 47–49. Diakses 17 Juli 2019. doi: 10.5152/eajm.2013.08.
Kurniasih, N, Kusmiyati, M, Nurhasanah, Sari, RP, Wafdan, R. (2015). Potensi Daun Sirsak ( Annona muricata Linn ), Daun Binahong ( Anredera cordifolia ( Ten ) Steenis ), dan Daun Benalu Mangga ( Dendrophthoe pentandra ) Sebagai Antioksidan Pencegah Kanker. Jurnal Edisis, IX(1), pp. 162–184. Diakses 17 Juli 2019. Available at: http://www.journal.uinsgd.ac.id/index.php/istek/article/viewFile/182/197.
Lenzen, S. (2008). The mechanisms of alloxan- and streptozotocin-induced diabetes, Diabetologia, 51(2), pp. 216–226. Diakses 17 Juli 2019. doi: 10.1007/s00125-007-0886-7.
McCance, KL, Huether, SE, Brashers, VL, and Rote, NS. (2015). Pathophysiology: The Biologic Basis For Disease In Adults And Children, 7th edn. ELSEVIER. Canada.
Mughal, MA, Aamir, K, Ali, M. (2019). The effects of glibenclamide on serum lipids and lipoproteins in type II non-insulin dependent diabetes mellitus. Journal of the Pakistan Medical Association, 49(4), pp. 89–92.
Perkumpulan Endorinologi Indonesia (PERKENI). (2015). Konsensus Pengendalian dan Pencegahan Diabetes Melitus Tipe 2 di Indonesia 2015. PERKENI. Jakarta. Diakses 17 Juli 2019. Available at: https://doi.org/10.1017/CBO9781107415324.004
Pietta, PG. (2000). Flavonoids as antioxidants. Journal of Natural Products, 63(7), pp. 1035–1042. Diakses 5 Juli 2019. doi: 10.1021/np9904509.
Rao, V, Sean, HT, Candasamy, M, Bhattamisra, SK. (2019). Diabetic nephropathy: An update on pathogenesis and drug development. Diabetes and Metabolic Syndrome: Clinical Research and Reviews. Elsevier Ltd, 13(1), pp. 754–762. doi: 10.1016/j.dsx.2018.11.054. Available from: https://www.sciencedirect.com/science/article/abs/pii/S1871402118305277?via%3Dihub
Sarian, MN, Ahmed, QU, So’ad, SZM. (2017). Antioxidant and antidiabetic effects of flavonoids: A structure-activity relationship based study. BioMed Research International. Hindawi. 2017. Diakses 17 Juli 2019. doi: 10.1155/2017/8386065.
Suryohudoyo, P. (1993). Oksidan, Antioksidan dan Radikal Bebas, Laboratorium Biokimia Fakultas Kedokteran Unair, pp. 1–11.
Tan, ALY, Forbes, JM and Cooper, ME. (2007). AGE, RAGE, and ROS in Diabetic Nephropathy, Seminars in Nephrology. W.B. Saunders, 27(2), pp. 130–143. Diakses 5 Juli 2019. doi: 10.1016/J.SEMNEPHROL.2007.01.006.
Yulianty, O, Sudiastuti, Nugroho. (2015). Efek Ekstrak Biji Ketumbar (Coriandrum sativum L.) terhadap Histologi Pankreas Mencit (Mus musculus L.) Diabetik Aloksan’, (November 2014).