PERBANDINGAN BPW DENGAN NaCl FISIOLOGIS 0,9% SEBAGAI LARUTAN PENGENCER TERHADAP TPC SUSU SAPI SEGAR

Abstract

Cow's milk is an important source of animal protein for the human body as it provides high protein and a good
growth medium for microorganisms. Milk that is milked in an improper way can contain microorganisms that
can reduce milk quality. One way to see contamination in fresh cow's milk is by Total Plate Count. Commonly
used diluent solutions are Buffer Peptone Water and 0.9% physiological NaCl. The purpose of the study was to
determine whether there is a difference between Buffer Peptone Water and 0.9% physiological NaCl as a
diluent solution against bacterial contamination based on the results of TPC examination. This study is an
experimental study with the use of BPW solution and 0.9% physiological NaCl. The results showed that the
average TPC value of fresh cow milk using BPW solvent in sample 1 was 1.36 x 107 CFU/mL, sample 2 was
1.25 x 107 CFU/mL, sample 3 was 1.12 x 107 CFU/mL. The results of the average TPC value of fresh cow's milk
using 0.9% NaCl solvent in sample 1 amounted to 2.75 x 105 CFU/mL, sample 2 amounted to 3 x 105 CFU/mL,
sample 3 amounted to 3.65 x 105 CFU/mL. Different solutions affect the results of the calculation of the number
of colonies in each cup counting method. The statistical results of the Paired Sample T-Test test obtained a
sigma value of sample 1 which is 0.127, sample 2 which is 0.137 and sample 3 which is 0.088 which means
there is no difference in the TPC test results of fresh cow's milk using BPW and 0.9% physiological NaCl.
Keywords: buffer peptone water; physiological NaCl 0.9%; fresh cow milk; total plate count.